Comparison of Ossification Process on Transverse Plane of Spine in Experimental Scoliosis

In the previous study, we have reported the relationship between IGF-1 and the experimental scoliosis chicks. In this study, we investigated the comparative study in ossification process of vertebrae growth between control and pinealectomized chicks before occurring of the scoliosis. Method: Chicks in 2days of age were pinealectomized, and after 4days, is which the scoliosis did not occur, were sacrificed. Sections of Th6 vertebrae in pinelectomized chicks were stained with hematoxiyllin-eosin, and ossification area were compared between control and pinelectomized chicks in transverse plane. Results: The ossification area of posterior side in pinealectomized chick’s vertebrae was significantly larger than control. The ossification area of anterior side showed same changes in both groups. We first reported that pinealectomy induced abnormal growth of arcus vertebrae in comparison with normal chick. We found the growth of spine in pinealectomized chick induced unbalance between anterior and posterior column before occurring of the scoliosis.